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2014/11/18 -It seems that blast reports the first best hit found in the database ONLY in the case where e-value and bit score from multiple hits are the ...

2018/9/28 -The parameter -max_target_seqs is set by default to 500. Does it mean that if there are more than 500 significant hits, I don't have a guarantee ...

2023/12/15 -A recent paper by Shah et al., 2018 explains the default behaviour in BLAST using the --max-target-seq setting. Many people use this setting ...

max_target_seqs. integer. 500. Number of aligned sequences to keep. Use with report formats that do not have separate definition line and alignment sections ...

NCBI blastp seems to have a bug where it reports different top hits when -max_target_seqs is changed. This is a serious problem because the first 20 hits ...

2018/9/25 -"BLAST documentation states "-max_target_seqs" parameter represents the "number of aligned sequences to keep". This statement is commonly ...

Figure 6 demonstrates the threshold capability of the BLAST-QC program. The first sequence is returned when ordering by evalue with the number of hits set to ...

ORF detection tool to find the correct frame. • ORF length. • HMM model trained on a set of genuine proteins (from the same species.

2018/9/24 -A common strategy involves using the '-max_target_seqs' parameter of the NCBI BLAST+ suite. According to the BLAST documentation itself (2008), ...

2021/8/1 -Database search heuristics implemented in the original blastn and megablast does not guarante that you will find the best hit if max_target_seqs ...