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Sound check and introduction
Overview for today
Quick overview of the project data
Adjusting the pipeline, input from the command line
Executing the pipeline for each SRA sample
Copy BAM files to windows using a shared folder in VirtualBox
Preparing a new R script, required packages for today
Building the exons per gene database
Adding the install script to the Github gist
Computing the length in base pairs of all transcripts
Loading the BAM alignment files into R
Extracting raw read counts from BAM files
Computing RPKM values in R
Violin plots of the RPKM distribution per sample
Quantile normalization & log2 transformation of RPKM values
Computing differential gene expression and fold change
Creating the volcano plot
Subset up/down regulated genes, a clustered heatmap
Creating an overview table for publication / supplemental files
biomaRt for gene annotation of up/down regulated genes
Data to Excel
Pathway over-representation analysis using DAVID
Microsoft Excel trick: Prevent genes turning into dates
Pathway over-representation analysis using DAVID (Continued)
Overview, Questions, and Outro