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Welcome Comments
Agenda
Investments in Beef Research in Canada
Achieving Reproductive Goals and Capitalizing on Reproductive Momentum Part 1
Polling the Audience
Achieving Reproductive Goals and Capitalizing on Reproductive Momentum Part 2
Questions and answers about calf crop percentages
Stay Connected with BCRC and Closing Comments
MTT stock solution : 5 mg/ml in D-PBS filtrated by 0.22 um filter
Remove 96 well plate from incubator and add 20 ul MTT stock solution to each well
Place 96 well plate into incubator at 37 C, 5% CO2 for 4 hours
Remove 96 well plate from incubator and aspirate the solution
Add 100 ul DMSO to each well
Rotate the plate for 5 min to distribute evenly
Absorbance is measured with a ELISA reader at 540 nm (reference absorbance value is 690 nm)
ELISA readout data