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Label the microtiter plate and transfer pipettes as noted in the product literature
Incubate for 5 minutes at room temperature
Wash the wells
Add reagents as outlined in the product literature
Incubate the plate for 15 minutes at 37 degrees Celsius
Add 100 μl of the secondary antibody to each of the wells
Prepare the detection substrate
Remove the secondary antibody solution from the wells using the transfer pipette designated for each sample
Add 100 μl of the substrate solution to each well
Examine Your Results