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1.Definition of toxicity and LD50 Value (Time line
2.Type of toxicity studies overview (Time line
3. Acute toxicity class method-OECD 423 (Time line
4. Comparison of OECD Guideline 420 i.e fixed dose procedure, 423 i.e Acute toxic class method and 425 i.e Up and down procedure (Time Line
5. Sighting study (Time Line
6. Observation Period (Time Line
7. Limit test (Time Line
8. Subacute and chronic toxicity test (Time line
9. Application of toxicity test (Time line
A. By Miller and Tenter method or by probit analysis (Time Line
B.By Karbers Method (Time Line.onward)