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therampegefromexiletribe | 238.9K views. Watch the latest videos about #therampegefromexiletribe on TikTok.
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2023/03/24Sections on Water- Using a smear of Wax (Ultramicrotomy)
Using a Smear of Wax on a Glass Knife
Carefully advance the Knife towards the Block until you can see both the knife and block.
Use the dial on the right C'Handle to move the Block. At the same time, move the left dial to advance the Knife towards the Block.
Move the Arm up and down slightly as you advance the Knife towards the Block
Move the left outer dial towards you to advance the Block towards the Knife by 0.5 micron.
Brush away any excess resin.
The wax holds the water above it & around the knife edge.
Keep the block face dry. Remove any water that adheres to the block face.
Pull sections off as they float on water.
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