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Transfer the sterilized tubing set to ColFeeder bottle with sterilized culture medium
Fill the bottle with 470ml culture medium
Lock the pump head onto the CelFeeder Pump module. Set the inoculation parameters and press start to begin the inoculation
Use the RATE keys to set the upward speed for the medium
Use the DELAY Keys to set the top holding time
Use the DELAY Keys to set the bottom holding time
Press START to begin the process
Medium change for perfusion culture
Stop CelFeeder pump, unmount the CelCradle bottle from the stage and the pumphead from the Celfeeder
Transfer the headplate to a CelFeeder bottle containing fresh culture medium
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