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Outline of the Course
Basic Principles in Electron Microscopy in an Electron Microscope
Types of Electron Microscopy Techniques
Conventional Methods
Advantages
Advantages of Coyotem in Ev Analysis
Limitations of Iotm in Ev Analysis
Immunogold Labeling in Cryotherium
Scanning Electron Microscopy Technique
Limitations of the Method
Welcome
What are extracellular vesicles?
Biological Activity
Evaluation of CFS performance
Extracellular Vesicle Composition
Extracellular vesicles as supportive lipid bilayers
Isolation of extracellular vesicles (EVs)
Are cell cultures good sources for the massive production or purification of EVs?
Is there any difference between the exosome isolation technique used with adherent cells compared with suspension cells?
Closing Remarks
Neurodegenerative Diseases
EVs and the nervous system
Prions: misfolded proteins with Infectious properties
Prions are associated with exosomes
Chemical alterations in exosome structure reduces infection efficiency
Do you need highly pure vesicles for RNA analysis?
Profiling miRNA expression in brain and serum of prion infected mice
Validation of prion EV miRNAs in human clinical sample
ALS BDEVs enriched in TDP-43
Acknowledgements
Background
Method Comparison Flow Chart
Results: Particle to protein ratio
Effect of PBS perfusion on brain-derived extracellular vesicles separation
Impact of post-mortem interval on brain relative protein content
Multidimensional scaling analysis
Cellular markers
Geo-ontology analysis
Q&A session
Is L1CAM really specific to neurons?
Opening Remarks
Role of Exosomes in Cell Migration
Exosome secretion and adhesion formation
Co-localization with ALIX and TSG101
Fusion events
Exosome secretion and angiogenesis
Exosomes induce reverse EphB signaling
Exosome knockdown does not affect tumor volume
Exosomes can amplify signals
Can exosomes seed pre-metastatic niches?