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ZymoPURE Plasmid Miniprep Protocol
Add 250 ul of ZymoPURE P1 (Red) to the bacterial cell pellet.
Immediately mix by gently inverting the tube 6-8 times. Do not vortex!
Mix thoroughly by inversion - do not vortex!
Centrifuge for 5 minutes at 16,000 xg.
Be careful not to disturb the yellow pellet and avoid transferring any cellular debris to the new tube.
Recommended Vacuum Protocol
With the vacuum off, add 800 ul of ZymoPURE Wash 2 to the Zymo-Spin II-P Column
Place the column in a Collection Tube and centrifuge at 10,000 xg for 1 minute in order to remove any residual wash buffer.
Store the eluted plasmid DNA at 5-20°C.
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