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Allow all reagents and samples to warm to room tempaerature before use
Vigorously agitate the Carbon Antigen for 20-30 seconds before use.
Dispense one free-falling drop (0.05 ml) of each control
Agitate the carbon antigen dispensing bottle, then dispense a few drops to make sure the needle passage is clear
Dispense one free-falling drop of the antigen suspension onto each sample while holding the bottle in a vertical position Do not restir the sample and antigen.
Following rotation, a brief hand rotation and Filting of the card should be performed.
After rotation, read results immediately under a high intensity lamp.