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The day before transfection, seed cells
Incubate overnight
Cells are 60-80% confluent at the time of transfection
Dilute DNA in jetPRIME buffer
Mix by vortexing quickly and briefly spinning down
Mix by quickly vortexing and briefly spinning down
Incubate at room temperature for 10 min.
Add transfection mix to cells in serum containing medium
Incubate 24 to 72 hours
Highly efficient in a wide variety of cell lines