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YouTube-Dr. K. PREM - PRIMER
this video describes how DNA footprinting assay could be a good assay to detect dna protein interaction.
YouTube-Animated biology With arpan
This video describes the method used to detect nucleosome free regions in chromatin. These DNase I hypersensitive sites are often in ...
YouTube-MCDB 427
Intro
The structure of chromatin
Experimental Setup
Mapping DNA that is not completely complexed with nucleosomes
Southern blot analysis
DNA with several hypersensitive sites
Mapping DNase I hypersensitive sites on chromatin
Mapping DNase I hypersensitive sites
Analysis of the data
Recap
This lecture is about the Therapeutic Enzymes and is focusing on two such enzymes; DNase I and Alginate Lyase, used for minimizing the ...
YouTube-Learn Biotechnology and Microbiology
Therapeutic Enzymes
Cystic Fibrosis Genetics
Cystic Fibrosis Pathogenesis
Chloride Concentration in Cystic Fibrosis
DNase I
Alginate Structure
Alginate Lyase Gene Isolation
Production of Alginate Lyase
Testing for Alginate Lyase Activity
Summary
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YouTube-National Human Genome Research Institute
A survey of chromatin accessibility across 400+ cell types and states
Components reflect distinct biological contexts and regulatory signal
GWAS signal is strongly enriched in DHS components
Extendability of the DHS annotation framework to new datasets
Acknowledgements
A short introduction to DNase I Hypersensitive sites and how this technique can be used to identify regulatory elements across the genome.
YouTube-Deborah Thurtle-Schmidt
Introduction
What are DNase I hypersensitive sites?
DNA is packaged in nucleosomes
Transcription factors
Nucleosomes are sensitive to nuclease digestion
Sequence analysis
Sequencing
Sequencing libraries
Mapping back to the genome
Applications of DNase-seq
In genetics, DNase I hypersensitive sites (DHSs) are regions of chromatin that are sensitive to cleavage by the DNase I enzyme.
YouTube-BMH learning
YouTube-Genetics
Nucleosome spacing
DNAseI digestion of chromatin
What is the biological significance of DNAseI hypersensitive sites?
Conclusion
Plant DHS database
DNAseI hypersensitive sites in different tissues
Nucleosome occupancy and position site preference
Histone acetylation
Basics of RDT part 14: Deoxyribonucleases (Examples-DNase I and S1 Nuclease) · Comments3.
YouTube-Instant Biology by Dr. Neelabh
Deoxyribonuclease
Function
Phosphodiester
Endo Deoxyribonuclease
Dna
Stabilization
Mg2 Plus
Labeling of Dna by Nick Translation
Glycosylation
Nicking
DNase-seq or (DNase I hypersensitive sites sequencing) .... Also called as DNase I footprinting assay... Its an in vitro method used to ...
What is DNase Seq?
Principle of DNase Seq
Footprint Strategy
DNA fragments are separated by polyacrylamide gel electrophoresis
DNaseI Footprinting is an important technique in molecular biology, as it allows researchers to find the exact DNA sequence bound by a ...
YouTube-Spartan Tutorials
19:07. Go to channel · Basics of RDT part 14: Deoxyribonucleases (Examples-DNase I and S1 Nuclease). Instant Biology by Dr. Neelabh•1.9K views.
YouTube-Dr. PREM-PRIMER Biotech Lectures
This video describes the DNase footprinting method. It includes information to identify which end of the DNA was labeled.
YouTube-{POSIT} Society
Each Band Represents a Nucleotide Difference in DNA
Examples of DNase Footprinting
How to Read the Gel
Limiting DNase Treatment
Example Data
Determining Nucleotides
The Sequence Covered by DNase
... with the DNA then DNAse I enzyme will fail to digest the region where the protein was interacting. This results in foot print on the gel.
YouTube-Quick Biochemistry Basics
Comments4 · DNAseI hypersensitive sites in chromatin · DNAse footprinting assay · STAT115 Chapter 13.5 Introduction to DNase-seq and ATAC-seq.
Dna Hypersensitivity Assay
Remove those System Histone Proteins
Restriction Enzyme Sites
Probe to the Dna
Conclusions
Zero Enzyme Control
A DNase footprinting assay is a DNA ... DNAse footprinting | how it works? ... Gene knock Out | Neomycin | G418- Casette I Experimental | PYQ ...
YouTube-BaaYo
... (DNase, for short) to cut the radioactively ... DNase will produce fragments, the smaller of ... I am sharing their material. © by original ...
YouTube-Shomu's Biology
Basic technique of DNase footprinting
DNase activity when DNA is bound with protein
Sequence specific DNA binding proteins protect sequence from digestion
Finding amount and size of protein
Fig 13.19 13.20 Mapping DNase I hypersensitive sites on chromatin ... DNase 1 Hypersensitive Sites | DHSs | DNase Seq |. BMH learning•2.4K views.
YouTube-Xiaole Shirley Liu
Chromatin Accessibility Profile
Combined Transcription Factor Density
Cutting Bias
Transcription Factor Binding
Dnase. 978 views · 1 year ago ...more. Abdelraouf Elmanama. 4.44K ... Basics of RDT part 14: Deoxyribonucleases (Examples-DNase I and S1 Nuclease).
YouTube-Abdelraouf Elmanama