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Surface Plasmon Resonance or SPR for short is an optical technology used to perform label-free biomolecular interaction analysis in real-time.
This fluidic-based design uses sensor chips which are addressed with the sample through a microfluidic system for a wide range of molecules.
SPR occurs when light moves from an optically dense medium to a less dense medium, such as from glass to air. It moves away from normal, and at an angle greater than the critical angle, it undergoes total internal reflection.
If a thin gold layer is placed on at the interface, then some of the reflected light is absorbed, reducing the intensity of reflected light at a specific angle known as the resonance angle.
This localization of these plasmons to the boundary and external medium makes surface plasmon resonance highly sensitive to any changes that occur within the field of the plasmon wave. Therefore, any change in the refractive index will result in a change of the SPR angle.
When a sample containing an analyte of interest is injected over the sensor chip, any binding of the analyte to immobilized ligands on the chip causes a change in the refractive index at the surface, …
resulting in a shift in the angle of total reflected light.
This shift can be monitored and plotted in real-time and is directly proportional to the amount of binding that occurs, …
allowing researchers to quantify the binding kinetics and affinity of the biomolecular interaction.
Throughput, flexibility, and sensitivity of the SPR platform gives researchers the potential to characterize biomolecular interactions in any binding study.
A wide range of molecules can be studied, from ions, fragments and small molecules to proteins and viruses.
Label-free biomolecular interaction analysis has revolutionized the field of antibody discovery and development. It has enabled researchers to assess the binding characteristics of potential therapeutic antibodies quickly and accurately - ultimately leading to significant advances in the development of such for more effective and specific biotherapeutics.
Sartorius – Simplifying Progress
The Sartorius label-free protein analysis portfolio has just expanded. In addition to our innovative industry standard fluidics-free biolayer interferometry technology, we have now added the first Octet® surface plasmon resonance instrument, the Octet® SF3 SPR.
Combining many of the features that researchers expect from BLI technology – like accuracy, precision, ease of use and simple maintenance – the Octet® SF3 offers a robust, high sensitivity, high throughput SPR alternative.
The Octet® SF3 is prepared for whatever challenge you take on, making use of a range of powerful attributes, including
The power of the Octet® SF3 also lies in its diverse range of injection types, from industry standard multi-cycle kinetics, to the patented OneStep®, OneStep® Two Comp, OneStep® High-Throughput, OneStep® Pulse and NeXtStep™ Gradient Injections.
OneStep® Gradient Injections are capable of creating an analyte gradient of at least three orders of magnitude. This is achieved by diffusing a single analyte concentration into a moving stream of buffer, which removes the need to create multiple dilution series.
This means you no longer need to spend time preparing multiple dilution series or worrying about inaccuracies in creating a specific analyte concentration series.
Instead, OneStep® Gradient Injections enable an accurate and comprehensive measurement of a molecule’s kinetics and affinity from a single analyte concentration in a single well. This means that analysis of a 96-well sample plate really does generate comprehensive data for 96 different samples. Imagine screening 768 unique compounds in a single unattended run – with no differences in results compared to multi-cycle kinetics – irrespective of the analyte concentration used!
After rapidly screening for molecules which warrant further investigation, it’s also important to understand their behavior across a range of different conditions.
And because samples can vary in size, shape and structure, their behavior under a range of conditions is also likely to differ considerably.
Competition assays are a critical component of the drug discovery process.
And to complete the package, an intuitive, user friendly acquisition and analysis platform is essential.
Whatever your project, assay, compound, or biologic of interest, the Octet® SF3 is powered and prepared for whatever challenge you take on.