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For ergonomic posture, adjust the viewing angle with the Ergo Wedge of the observation system.
Orientate knife in the east-west direction using the E-W coarse wheel.
For precise approach, switch on the backlight only
Move the sample below the knife.
Make sure the knife edge is well contacted with water.
At the first section reduce speed 2mm/sec and feed 30nm
Manipulate sections with an eyelash.
Pick up ribbon with the perfect loop
Remove water using a filter paper
Store grid in a grid-box.