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Why Are We Interested in Predicting Substrates for Enzymes
General Prediction Model
General Prediction Pipeline
Recap of the Steps
Sampling Strategies
Model Architecture
Do You Think Balancing the Data Could Have Improved the Model Performance
Does Your Model Consider Enzyme Complexes At All
Is the Model Only Suitable for Predicting the Interaction with Substrates or with Co-Actors As Well for Example Can It Tell the Difference between Atp or Gtp Dependent Enzymes
Can Active Sites or Catalytic Residues although Sparsely Available Be Utilized To Improve Model Accuracy or Interpretability
Introduction
What is mushroom substrate?
Lignin, cellulose & hemicellulose
Nitrogen
Small amount of minerals K, P, Mg, Ca, S
Ph Level 5 - 6.5
Good structure for air exchange
Moisture content 50 - 70
Absence of competitor organisms
Sterilisation vs Pasteurisation
Popular Substrate Options
Straw
Other agricultural byproducts
Sawdust
Sawdust pellets or straw pellets
Manure
Coco coir & vermiculite
Coffee grounds
Cardboard
Supplementation
Hardwood logs
Woodchip beds
Deciding on your substrate
Low tech mushroom substrate
Masters Mix