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2023/04/08Prepare and handle your dna samples
Measurement technique
Choose the appropriate protocol for dna isolation
Add concentrated sodium chloride
Wash the beads with ethanol
Move it to deep storage at minus 20 celsius
Take 200 microliters of whole blood
Adding your whole genomic dna sample
Identify the percentage of single-stranded dna
Fill out a brief survey at the end of this session
Optimizing the Erythrocyte (RBC) Lysis Process
Effectively Carrying Out Erythrocyte Lysis: Frozen Blood Samples
Ensuring Proper Removal of Supernatant after RBC Lysis
Ensure Complete Resuspension of Leukocyte Pellet at Each Resuspension Step
Complete resuspension is particularly important during nuclei prep and lysis.